Common Mistakes in Operating a High Pressure Autoclave

Overloading for Efficiency

Many people, in an attempt to save time, stuff all sterilization items into the high pressure autoclave without considering the proper spatial arrangement, resulting in overloading. It's essential to leave enough space between items to allow steam to circulate freely. When test tubes or petri dishes are stacked in a way that obstructs steam flow, the temperature in the center may drop as low as 80°C. Uneven steam penetration can significantly reduce the sterilization efficiency of the high pressure autoclave.

Ignoring "Cold Air Exhaust"

Before sterilization, it's crucial to expel all cold air from the high pressure autoclave. Cold air can negatively affect steam penetration, leading to incomplete sterilization. If you notice that the cold air is not being expelled properly, be sure to check if the exhaust vents are clogged, as this can allow bacteria to survive.

Operating the Autoclave with Malfunctions

Like all equipment, the high pressure autoclave has a limited lifespan. If you notice any issues, such as aging seals, inaccurate pressure gauges, or faulty valves, do not continue using it. Timely maintenance and part replacement are essential to ensure the proper functioning of the high pressure autoclave and to avoid potential safety hazards.

Neglecting Regular Maintenance and Cleaning

If the high pressure autoclave is not regularly maintained, its performance will deteriorate over time. Problems like worn seals, faulty pressure gauges, and clogged drainage ports will all affect the sterilization results and may even cause the autoclave to fail. Additionally, if the same water is reused repeatedly, it can accumulate dirt, clog the solenoid valve, and even cause the heating element to rust or get damaged.

It is recommended to replace the water at least once a week, and if the sterilized items contain slight corrosive materials, it is advisable to change the water daily. The sterilization chamber should also be cleaned regularly to remove scale and debris.

Neglecting Sterilization Effect Monitoring

Every sterilization cycle should include the use of a chemical indicator card or strip to monitor the success of the process. The chemical indicator will change color according to the sterilization conditions (such as temperature, time, and pressure). If the indicator does not change color or only partially changes color, this suggests that the sterilization process has failed, and the cycle must be repeated.